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KMID : 1123920120260060908
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Korean Journal of Oriental Physiology and Pathology
2012 Volume.26 No. 6 p.908 ~ p.914
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Study on Comparison of Ingredient Quantities and Biological Activities of Galgeun-tang according to Extraction Solvent
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Kim Ye-Ji
Kim Ohn-Soon
Seo Chang-Seob
Lim Hye-Sun
Yoo Sae-Room
Jeon Woo-Young
Jin Seong-Eun
Shin In-Sik
Kim Jung-Hoon
Shin Na-Ra
Kim Seong-Sil
Lee Mee-Young
Jeong Soo-Jin
Ha Hye-Kyung
Shin Hyeun-Kyoo
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Abstract
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To investigate the difference and change of ingredient and efficacy of Galgeun-tang (GGT) according to extraction solvent, water and 70% EtOH, the quantities of index components and the several in vitro activities of two kinds of GGT extract were compared. The contents of extracts were analyzed with HPLC. The biological activities such as anti-inflammatory and anti-obesity effects were measured through cell line-based in vitro assay. The cytotoxicity was measured by CCK-8 assay in RAW 264.7 cell, BEAS-2B cell, HaCaT cell and 3T3-L1 cell. We compared effects of two kinds of extract by measuring NO, PGE2, IL-6 and TNF-α in RAW264.7 cell, RANTES in BEAS-2B cell, MDC and RANTES in HaCaT cell and GPDH activity and leptin level in differentiated 3T3-L1. 70% EtOH extract of GGT contained more compositions than water extract. The inhibitory effect of water extract of GGT on NO in RAW 264.7 cell and GPDH activity in 3T3-L1 was stronger than that of 70% EtOH. 70% EtOH has a stronger inhibitory effect on PGE2 in RAW264.7 cell, RANTES in BEAS-2B cell, MDC, RANTES in HaCaT cell, leptin in 3T3-L1 cell than water extraction. These results suggest that the ingredient and efficacy from 70% EtOH extract of GGT are more effective than water extract.
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KEYWORD
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Galgeun-tang(Gegen-tang), extraction solvent, ingredient analysis, biological activities
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